Estimation Model Research Articles

Decomposition in an extreme cold environment and associated microbiome—prediction model implications for the postmortem interval estimation

IntroductionThe accurate estimation of postmortem interval (PMI), the time between death and discovery of the body, is crucial in forensic science investigations as it impacts legal outcomes. PMI estimation in extremely cold environments becomes susceptible to errors and misinterpretations, especially with prolonged PMIs. This study addresses the lack of data on decomposition in extreme cold by providing the first overview of decomposition in such settings. Moreover, it proposes the first postmortem microbiome prediction model for PMI estimation in cold environments, applicable even when the visual decomposition is halted.MethodsThe experiment was conducted on animal models in the second-coldest region in the United States, Grand Forks, North Dakota, and covered 23 weeks, including the winter months with temperatures as low as −39°C. Random Forest analysis models were developed to estimate the PMI based either uniquely on 16s rRNA gene microbial data derived from nasal swabs or based on both microbial data and measurable environmental parameters such as snow depth and outdoor temperatures, on a total of 393 samples.ResultsAmong the six developed models, the best performing one was the complex model based on both internal and external swabs. It achieved a Mean Absolute Error (MAE) of 1.36 weeks and an R2 value of 0.91. On the other hand, the worst performing model was the minimal one that relied solely on external swabs. It had an MAE of 2.89 weeks and an R2 of 0.73. Furthermore, among the six developed models, the commonly identified predictors across at least five out of six models included the following genera: Psychrobacter (ASV1925 and ASV1929), Carnobacterium (ASV2872) and Pseudomonas (ASV1863).DiscussionThe outcome of this research provides the first microbial model able to predict PMI with an accuracy of 9.52 days over a six-month period of extreme winter conditions.

Sometimes simple is good enough: An analysis of methods for residential building population estimation

AbstractResidential building population data can be useful in a breadth of urban planning, health, transportation, and business applications. Unfortunately, complete datasets of residential building populations are not widely available for use in Canada, and therefore either larger census geographies are used or residential building populations must be estimated. This research explores four different methods of estimating residential building populations, including: an equal allocation method, two measures based on building volume, and a novel method that integrates census data at the dissemination area level to calibrate a population estimation model. This work comprises three parts: 1) a description of these approaches, 2) an evaluation of their validity in a case study in Hamilton, Ontario, and 3) an application of these methods in measuring spatial accessibility to schools. Our results show that most methods yield very similar results, and most provide reasonable estimates of building populations that could be useful for some analytical tasks. However, all methods resulted in instances of error, particularly for the largest population buildings. We conclude that while more complex methods do not significantly outperform simpler methods based on building volume alone, the blend of these methods could yield more accurate population predictions.

Combining spectrum, thermal, and texture features using machine learning algorithms for wheat nitrogen nutrient index estimation and model transferability analysis

The nitrogen nutrition index (NNI) has been extensively applied for the diagnosis of crop nitrogen status, providing insights into efficient nitrogen utilization and plant growth. In this study, we utilized a low-altitude unmanned aerial vehicle (UAV) platform, equipped with multispectral (MS), red–green–blue (RGB), and thermal infrared (TIR) cameras, to comprehensively capture wheat spectral information. The analysis of the relationship between NNI and relative yield revealed an initially linear relationship, which saturated for high NNI values. To enhance accuracy and minimize complexity, we employed a random forest (RF) – recursive feature elimination (RFE) method to select features as inputs for four machine learning (ML) models: back propagation neural network (BPNN), extreme learning machine (ELM), support vector regression (SVR), and Gaussian process regression (GPR). After feature selection, the prediction accuracies of single-sensor models were ranked as: MS > RGB > TIR. The R2 values for the four ML models were in the range of 0.54–0.75. Among multi-sensor combinations, the GPR with MS + RGB + TIR input features achieved the best results with R2 = 0.89 and RPD = 2.52. Further, the dataset was partitioned into six subsets based on location and cultivar variety to evaluate model transferability. The results showed that the transferability largely suffered during the bivariate conditions of different varieties at different locations; the transferability of the model was average improved by 11 % when GPR was combined with transfer component analysis (TCA). The accuracy and transferability of the NNI estimation models significantly improved, offering valuable guidance and methodological support for diagnosing the nitrogen nutrient status of wheat.

A CNN Model for Physical Activity Recognition and Energy Expenditure Estimation from an Eyeglass-Mounted Wearable Sensor

Metabolic syndrome poses a significant health challenge worldwide, prompting the need for comprehensive strategies integrating physical activity monitoring and energy expenditure. Wearable sensor devices have been used both for energy intake and energy expenditure (EE) estimation. Traditionally, sensors are attached to the hip or wrist. The primary aim of this research is to investigate the use of an eyeglass-mounted wearable energy intake sensor (Automatic Ingestion Monitor v2, AIM-2) for simultaneous recognition of physical activity (PAR) and estimation of steady-state EE as compared to a traditional hip-worn device. Study data were collected from six participants performing six structured activities, with the reference EE measured using indirect calorimetry (COSMED K5) and reported as metabolic equivalents of tasks (METs). Next, a novel deep convolutional neural network-based multitasking model (Multitasking-CNN) was developed for PAR and EE estimation. The Multitasking-CNN was trained with a two-step progressive training approach for higher accuracy, where in the first step the model for PAR was trained, and in the second step the model was fine-tuned for EE estimation. Finally, the performance of Multitasking-CNN on AIM-2 attached to eyeglasses was compared to the ActiGraph GT9X (AG) attached to the right hip. On the AIM-2 data, Multitasking-CNN achieved a maximum of 95% testing accuracy of PAR, a minimum of 0.59 METs mean square error (MSE), and 11% mean absolute percentage error (MAPE) in EE estimation. Conversely, on AG data, the Multitasking-CNN model achieved a maximum of 82% testing accuracy in PAR, a minimum of 0.73 METs MSE, and 13% MAPE in EE estimation. These results suggest the feasibility of using an eyeglass-mounted sensor for both PAR and EE estimation.

Modeling Forest Above-Ground Biomass of Teak (Tectona grandis L. F.) Using Field Measurement and Sentinel-2 Imagery

Over the last few decades, remote sensing has emerged as a dependable and cost-effective method for collecting precise data on forest biophysical parameters, aiding in sustainable forest management and global initiatives to combat climate change. This research aimed to develop a model for estimating the above-ground biomass (AGB) of Teak (Tectona grandis L. F.) by combining field measurements with Sentinel-2 earth observation data. The study took place in 36-year-old teak plantation areas within the Sagarnath Forest Development Project in Nepal’s Sarlahi district. Field measurements were conducted using a destructive systematic sampling method, employing 10 × 10 m2 sample plots, and the volume of logs was determined using Newton’s formula. A total of 30 sample plots were used for calibration, while 10 were utilized for validation purposes. The findings revealed that the average AGB per plot was 814 kg (equivalent to 81.4 t ha−1), with a minimum value of 716 kg (71.6 t ha−1) and a maximum value of 1,060 kg (106 t ha−1). The study utilized five independent variables, namely, the Red band, Green band, Blue band, near-infrared (NIR), and normalized difference vegetation index (NDVI) values from Sentinel-2 imagery data, to develop estimation models. Among the 12 models examined, model M10 proved to be the best fit for accurate AGB estimation (adjusted R2 = 0.9809, RMSE = 0.01269, AIC = −170, and p-value = < 8.39e−21). The equation of the best-fitted model was ln (AGB) = A + B × Red + C × Green + D × Blue2 + E × ln (NIR) + F × ln (NDVI), providing an accurate estimate of AGB. Model validation involved a t-test comparing the observed and calculated AGB values for ten sample plots, demonstrating no significant difference (p-value = 0.3662 > 0.05). This model has the potential to facilitate AGB biomass calculations and carbon stock estimates for teak plantations of similar age groups.

Appropriate cumulative fatigue damage models for fatigue life estimation applied to high-strength steels

Appropriate cumulative fatigue damage models for fatigue life estimation applied to high-strength steels

SPICER: Self-supervised learning for MRI with automatic coil sensitivity estimation and reconstruction.

To introduce a novel deep model-based architecture (DMBA), SPICER, that uses pairs of noisy and undersampled k-space measurements of the same object to jointly train a model for MRI reconstruction and automatic coil sensitivity estimation. SPICER consists of two modules to simultaneously reconstructs accurate MR images and estimates high-quality coil sensitivity maps (CSMs). The first module, CSM estimation module, uses a convolutional neural network (CNN) to estimate CSMs from the raw measurements. The second module, DMBA-based MRI reconstruction module, forms reconstructed images from the input measurements and the estimated CSMs using both the physical measurement model and learned CNN prior. With the benefit of our self-supervised learning strategy, SPICER can be efficiently trained without any fully sampled reference data. We validate SPICER on both open-access datasets and experimentally collected data, showing that it can achieve state-of-the-art performance in highly accelerated data acquisition settings (up to ). Our results also highlight the importance of different modules of SPICER-including the DMBA, the CSM estimation, and the SPICER training loss-on the final performance of the method. Moreover, SPICER can estimate better CSMs than pre-estimation methods especially when the ACS data is limited. Despite being trained on noisy undersampled data, SPICER can reconstruct high-quality images and CSMs in highly undersampled settings, which outperforms other self-supervised learning methods and matches the performance of the well-known E2E-VarNet trained on fully sampled ground-truth data.

Neoadjuvant chemotherapy is associated with improved disease-free survival in pancreatic cancer patients undergoing pancreaticoduodenectomy with vascular resection.

Neoadjuvant chemotherapy (NAC) is becoming favored for all pancreatic adenocarcinoma (PDAC). Patients with seemingly resectable disease infrequently still display vascular involvement intraoperatively. Outcomes following NAC versus upfront surgery in patients undergoing pancreaticoduodenectomy (PD) with vascular resection are unknown. We performed a retrospective cohort study of PDAC patients who underwent PD with vascular resection between January 1, 2013, to December 31, 2020, within a single academic center. Clinicopathologic characteristics and disease-free survival (DFS) were compared between NAC versus upfront surgery cohorts using the Kaplan-Meier estimate and Cox proportional-hazards regression model. Eighty-one patients who underwent PD with vascular resection for PDAC were included. Forty-six patients (56%) received NAC. The NAC cohort more often had pathologic N0 status (47.8% vs. 8.6%, p < 0.001), had decreased vascular invasion (11% vs. 40%, p = 0.002), and completed chemotherapy (80% vs. 40%, p < 0.01). The NAC cohort demonstrated improved DFS (40.5 vs. 14.3 months, p = 0.007). In multivariable analysis, NAC remained independently associated with increased DFS (HR = 0.48, p = 0.02). NAC was associated with improved clinicopathologic outcomes and DFS in PD with vascular resection. These findings demonstrate the advantage of NAC in PDAC patients undergoing PD with vascular resection.

Phenotyping the Anthocyanin Content of Various Organs in Purple Corn Using a Digital Camera

Anthocyanins are precious industrial raw materials. Purple corn is rich in anthocyanins, with large variation in their content between organs. It is imperative to find a rapid and non-destructive method to determine the anthocyanin content in purple corn. To this end, a field experiment with ten purple corn hybrids was conducted, collecting plant images using a digital camera and determining the anthocyanin content of different organ types. The average values of red (R), green (G) and blue (B) in the images were extracted. The color indices derived from RGB arithmetic operations were applied in establishing a model for estimation of the anthocyanin content. The results showed that the specific color index varied with the organ type in purple corn, i.e., ACCR for the grains, BRT for the cobs, ACCB for the husks, R for the stems, ACCB for the sheaths and BRT for the laminae, respectively. Linear models of the relationship between the color indices and anthocyanin content for different organs were established with R2 falling in the range of 0.64–0.94. The predictive accuracy of the linear models, assessed according to the NRMSE, was validated using a sample size of 2:1. The average NRMSE value was 11.68% in the grains, 13.66% in the cobs, 8.90% in the husks, 27.20% in the stems, 7.90% in the sheaths and 15.83% in the laminae, respectively, all less than 30%, indicating that the accuracy and stability of the model was trustworthy and reliable. In conclusion, this study provided a new method for rapid, non-destructive prediction of anthocyanin-rich organs in purple corn.

Combining features selection strategy and features fusion strategy for SPAD estimation of winter wheat based on UAV multispectral imagery

The Soil Plant Analysis Development (SPAD) is a vital index for evaluating crop nutritional status and serves as an essential parameter characterizing the reproductive growth status of winter wheat. Non-destructive and accurate monitorin3g of winter wheat SPAD plays a crucial role in guiding precise management of crop nutrition. In recent years, the spectral saturation problem occurring in the later stage of crop growth has become a major factor restricting the accuracy of SPAD estimation. Therefore, the purpose of this study is to use features selection strategy to optimize sensitive remote sensing information, combined with features fusion strategy to integrate multiple characteristic features, in order to improve the accuracy of estimating wheat SPAD. This study conducted field experiments of winter wheat with different varieties and nitrogen treatments, utilized UAV multispectral sensors to obtain canopy images of winter wheat during the heading, flowering, and late filling stages, extracted spectral features and texture features from multispectral images, and employed features selection strategy (Boruta and Recursive Feature Elimination) to prioritize sensitive remote sensing features. The features fusion strategy and the Support Vector Machine Regression algorithm are applied to construct the SPAD estimation model for winter wheat. The results showed that the spectral features of NIR band combined with other bands can fully capture the spectral differences of winter wheat SPAD during the reproductive growth stage, and texture features of the red and NIR band are more sensitive to SPAD. During the heading, flowering, and late filling stages, the stability and estimation accuracy of the SPAD model constructed using both features selection strategy and features fusion strategy are superior to models using only a single feature strategy or no strategy. The enhancement of model accuracy by this method becomes more significant, with the greatest improvement observed during the late filling stage, with R2 increasing by 0.092-0.202, root mean squared error (RMSE) decreasing by 0.076-4.916, and ratio of performance to deviation (RPD) increasing by 0.237-0.960. In conclusion, this method has excellent application potential in estimating SPAD during the later stages of crop growth, providing theoretical basis and technical support for precision nutrient management of field crops.

Monitoring of Chlorophyll Content of Potato in Northern Shaanxi Based on Different Spectral Parameters

Leaf chlorophyll content (LCC) is an important physiological index to evaluate the photosynthetic capacity and growth health of crops. In this investigation, the focus was placed on the chlorophyll content per unit of leaf area (LCCA) and the chlorophyll content per unit of fresh weight (LCCW) during the tuber formation phase of potatoes in Northern Shaanxi. Ground-based hyperspectral data were acquired for this purpose to formulate the vegetation index. The correlation coefficient method was used to obtain the “trilateral” parameters with the best correlation between potato LCCA and LCCW, empirical vegetation index, any two-band vegetation index constructed after 0–2 fractional differential transformation (step size 0.5), and the parameters with the highest correlation among the three spectral parameters, which were divided into four combinations as model inputs. The prediction models of potato LCCA and LCCW were constructed using the support vector machine (SVM), random forest (RF) and back propagation neural network (BPNN) algorithms. The results showed that, compared with the “trilateral” parameter and the empirical vegetation index, the spectral index constructed by the hyperspectral reflectance after differential transformation had a stronger correlation with potato LCCA and LCCW. Compared with no treatment, the correlation between spectral index and potato LCC and the prediction accuracy of the model showed a trend of decreasing after initial growth with the increase in differential order. The highest correlation index after 0–2 order differential treatment is DI, and the maximum correlation coefficients are 0.787, 0.798, 0.792, 0.788 and 0.756, respectively. The maximum value of the spectral index correlation coefficient after each order differential treatment corresponds to the red edge or near-infrared band. A comprehensive comparison shows that in the LCCA and LCCW estimation models, the RF model has the highest accuracy when combination 3 is used as the input variable. Therefore, it is more recommended to use the LCCA to estimate the chlorophyll content of crop leaves in the agricultural practices of the potato industry. The results of this study can enhance the scientific understanding and accurate simulation of potato canopy spectral information, provide a theoretical basis for the remote sensing inversion of crop growth, and promote the development of modern precision agriculture.

Increasing the performance of a hospital department with budget allocation model and machine learning assisted by simulation

ABSTRACT The COVID-19 pandemic highlighted the critical need for efficient resource management in healthcare. In this study, the internal medicine outpatient clinic in a hospital is modelled by simulation method. Appropriate statistical distributions of the parameters are derived from past data. The results of a limited number of simulation runs are used as training data for machine learning techniques and an estimation model is selected among them. The estimation results are considered as input to a mathematical model which determines the optimal budget allocation for improving the system performance. Analysis considers patient waiting times and system throughput under varied parameters. A significant amount of time is saved by using machine learning to predict the simulation model outcomes, which had previously taken a total of around 7 hours reduced to 30–40 minutes. Time savings through machine learning are projected to be notably greater for more complex simulations comparing to current case.

Learning to Choose: Behavioral Dynamics Underlying the Initial Acquisition of Decision Making

Current theories of decision making propose that decisions arise through competition between choice options. Computational models of the decision process estimate how quickly information about choice options is integrated and how much information is needed to trigger a choice. Experiments using this approach typically report data from well-trained participants. As such, we do not know how the decision process evolves as a decision-making task is learned for the first time. To address this gap, we used a behavioral design separating learning the value of choice options from learning to make choices. We trained male rats to respond to single visual stimuli with different reward values. Then, we trained them to make choices between pairs of stimuli. Initially, the rats responded more slowly when presented with choices. However, as they gained experience in making choices, this slowing reduced. Response slowing on choice trials persisted throughout the testing period. We found that it was specifically associated with increased exponential variability when the rats chose the higher value stimulus. Additionally, our analysis using drift diffusion modeling revealed that the rats required less information to make choices over time. These reductions in the decision threshold occurred after just a single session of choice learning. These findings provide new insights into the learning process of decision-making tasks. They suggest that the value of choice options and the ability to make choices are learned separately, and that experience plays a crucial role in improving decision-making performance.Significance StatementWe investigated the dynamics of decision-making as rats initially learned to choose between visual stimuli associated with different rewards. Unlike prior research focusing on well-trained participants, we explored the initial stages of learning to make decisions. We used a behavioral design that separated value learning from choice learning. Initially, rats exhibited slower responses when making choices, but this slowing diminished with experience. Response slowing persisted throughout the period of early choice learning. Drift diffusion modeling found reduced evidence of reduced information requirements for making choices over the period of early learning, with decision thresholds decreasing after just one choice-learning session. These studies revealed that experience significantly enhances decision-making, and shed light on the learning mechanisms that underlie decision-making tasks.

Normalised difference moisture index in water stress assessment of maize crops

Remote sensing is a promising technique for better management of water resources in agriculture through improvement of dynamic control and operational scheduling on irrigated croplands. The main goal of this study was to identify the possibilities of application of the normalised difference moisture index (NDMI) to water stress monitoring in maize crops, and to determine the relationship between the index and soil moisture content. The study was carried out in 2019–2021 in the experimental fields of disturbed maize, cultivated on dark-chestnut soils in the Southern Ukraine at the NAAS Institute of Climate-Smart Agriculture. The crop cultivation technology was common for the conditions of the steppe zone of Ukraine. Actual soil moisture content was determined by gravimetric method in the pre-sowing and post-harvest period. The NDMI values were calculated using cloudless aerospace images from the satellites Landsat 8, Sentinel-2, and MODIS with 250 m resolution. It was revealed that the seasonal NDMI dynamics perfectly reflected the water-supply conditions of the disturbed maize, and could be used for operational monitoring and scheduling of irrigation. The parameters of the water-supply conditions were determined in 2021, which was the wettest year of the study: the cumulative seasonal NDMI reached 1.71, while the highest water stress was recorded in the driest year, 2020, – the cumulative NDMI was 0.15. Additionally, there was a moderately strong negative correlation between NDMI and soil moisture content, and the coefficient of determination was 0.62. The linear regression models, developed to predict soil moisture content in the 0–100 cm layer depending on the NDMI values, had good fitting quality and reasonable accuracy, but they required further calibration and extension of the initial dataset to provide more robust and reliable results for practical implementation. Based on the results of the study, spatial NDMI could be considered a good and reliable tool for improving irrigation water management. Further studies should focus on the practical implementation of the NDMI-based model of moisture-content estimation, as well as on the possibilities of the index usage for mapping irrigated lands.

Using frequency domain analysis to elucidate travel time reliability along congested freeway corridors

Travel time reliability (TTR) is an essential measure of service for traffic performance management, especially for congested freeway corridors. This paper proposes a systematic analytical framework to analyze the predictable and unpredictable variations in route TTR in corridor networks. More specifically, the predictable variation in route TTR is estimated with a deterministic fluid-based polynomial arrival queue (PAQ) model, while the unpredictable variation in route TTR is analyzed through the residual induced by the PAQ estimation model. Based on the output of the PAQ estimation model, a frequency-domain approach is proposed to decompose the observed time-domain travel time into underlying unobserved factors in different temporal resolutions. With the discrete-time Fourier transform method and the Butterworth low-pass filtering technique, it is capable of analytically uncovering different temporal scales of predictable variations in TTR, including the trend of day-to-day variations, the dynamics of within-day variations, as well as the stochasticity of within-period variations. Connecting with the unpredictable variations represented by the residual due to the PAQ approximation model, we can comprehensively elucidate TTR for congested freeway corridors. Case studies are conducted to show the effectiveness of the proposed frequency-domain approach in elucidating and measuring different contributing elements in TTR, and more specifically, the four components, i.e., the trend of day-to-day variations, the dynamics of within-day variations, the stochasticity of within-period variations, and the residual induced by the PAQ estimation model, account for 64.9 %, 20.2 %, 1.2 %, 13.7 % of variations in the route travel time, respectively, based on a case study with the PeMS dataset.

Hyperspectral estimation model of soil organic matter content based on principal gradient grey information

PurposeIn order to explore a new estimation approach of hyperspectral estimation, this paper aims to establish a hyperspectral estimation model of soil organic matter content with the principal gradient grey information based on the grey information theory.Design/methodology/approachFirstly, the estimation factors are selected by transforming the spectral data. The eigenvalue matrix of the modelling samples is converted into grey information matrix by using the method of increasing information and taking large, and the principal gradient grey information of modelling samples is calculated by using the method of pro-information interpolation and straight-line interpolation, respectively, and the hyperspectral estimation model of soil organic matter content is established. Then, the positive and inverse grey relational degree are used to identify the principal gradient information quantity of the test samples corresponding to the known patterns, and the cubic polynomial method is used to optimize the principal gradient information quantity for improving estimation accuracy. Finally, the established model is used to estimate the soil organic matter content of Zhangqiu and Jiyang District of Jinan City, Shandong Province.FindingsThe results show that the model has the higher estimation accuracy, among the average relative error of 23 test samples is 5.7524%, and the determination coefficient is 0.9002. Compared with the commonly used methods such as multiple linear regression, support vector machine and BP neural network, the hyperspectral estimation accuracy of soil organic matter content is significantly improved. The application example shows that the estimation model proposed in this paper is feasible and effective.Practical implicationsThe estimation model in this paper not only fully excavates and utilizes the internal grey information of known samples with “insufficient and incomplete information”, but also effectively overcomes the randomness and grey uncertainty in the spectral estimation. The research results not only enrich the grey system theory and methods, but also provide a new approach for hyperspectral estimation of soil properties such as soil organic matter content, water content and so on.Originality/valueThe paper succeeds in realizing both a new hyperspectral estimation model of soil organic matter content based on the principal gradient grey information and effectively dealing with the randomness and grey uncertainty in spectral estimation.

A Cognitive Approach for Modeling Customer Demand Dynamics for Optimal Product Release Strategies

Abstract As agile processes are increasingly adopted for product design and as consumer preferences are rapidly evolving with increasing information available from digital media, there is a need for a demand model that can accommodate the dynamics of product development. However, existing models of demand estimation, such as the discrete-choice models, do not capture the dynamics of product development and decision-making processes and thus are unable to effectively capture the effect of product updates and the release of information. To address this gap, we present a dynamic demand model and demonstrate how it can be used to determine the optimal time to release product updates. The demand model is based on decision field theory (DFT), which enables the modeling of the dynamic behavior of human decision makers. The contributions of this paper are as follows. First, we formulate a computational model for demand modeling built on DFT and demonstrate the viability of using the model to determine product release strategies. Second, we provide analytical approximations of the demand model and compare the accuracy of the approximated demand against the demand predicted by the dynamics model. Third, we show an example of a game played by competitors trying to optimize demand for their products by choosing the optimal update time relative to each other. Finally, we demonstrate the feasibility of parameter estimation using only the demand data.

Evaluating the utility of ZNF331 promoter methylation as a prognostic and predictive marker in stage III colon cancer: results from CALGB 89803 (Alliance)

ABSTRACT While epigenomic alterations are common in colorectal cancers (CRC), few epigenomic biomarkers that risk-stratify patients have been identified. We thus sought to determine the potential of ZNF331 promoter hypermethylation (mZNF331) as a prognostic and predictive marker in colon cancer. We examined the association of mZNF331 with clinicopathologic features, relapse, survival, and treatment efficacy in patients with stage III colon cancer treated within a randomized adjuvant chemotherapy trial (CALGB/Alliance89803). Residual tumour tissue was available for genomic DNA extraction and methylation analysis for 385 patients. ZNF331 promoter methylation status was determined by bisulphite conversion and fluorescence-based real-time polymerase chain reaction. Kaplan-Meier estimator and Cox proportional hazard models were used to assess the prognostic and predictive role of mZNF331 in this well-annotated dataset, adjusting for clinicopathologic features and standard molecular markers. mZNF331 was observed in 267/385 (69.4%) evaluable cases. Histopathologic features were largely similar between patients with mZNF331 compared to unmethylated ZNF331 (unmZNFF31). There was no significant difference in disease-free or overall survival between patients with mZNF331 versus unmZNF331 colon cancers, even when adjusting for clinicopathologic features and molecular marker status. Similarly, there was no difference in disease-free or overall survival across treatment arms when stratified by ZNF331 methylation status. While ZNF331 promoter hypermethylation is frequently observed in CRC, our current study of a small subset of patients with stage III colon cancer suggests limited applicability as a prognostic marker. Larger studies may provide more insight and clarity into the applicability of mZNF331 as a prognostic and predictive marker.

Non-Contact Tilapia Mass Estimation Method Based on Underwater Binocular Vision

The non-destructive measurement of fish is an important link in intelligent aquaculture, and realizing the accurate estimation of fish mass is the key to the stable operation of this link. Taking tilapia as the object, this study proposes an underwater tilapia mass estimation method, which can accurately estimate the mass of free-swimming tilapia under non-contact conditions. First, image enhancement is performed on the original image, and the depth image is obtained by correcting and stereo matching the enhanced image using binocular stereo vision technology. And the fish body is segmented by an SAM model. Then, the segmented fish body is labeled with key points, thus realizing the 3D reconstruction of tilapia. Five mass estimation models are established based on the relationship between the body length and the mass of tilapia, so as to realize the mass estimation of tilapia. The results showed that the average relative errors of the method models were 5.34%~7.25%. The coefficient of determination of the final tilapia mass estimation with manual measurement was 0.99, and the average relative error was 5.90%. The improvement over existing deep learning methods is about 1.54%. This study will provide key technical support for the non-destructive measurement of tilapia, which is of great significance to the information management of aquaculture, the assessment of fish growth condition, and baiting control.

Method for Monitoring Wheat Growth Status and Estimating Yield Based on UAV Multispectral Remote Sensing

An efficient and accurate estimation of wheat growth and yield is important for wheat assessment and field management. To improve the accuracy and stability of wheat growth and yield estimation, an estimation method based on a genetic algorithm-improved support vector regression (GA-SVR) algorithm was proposed in this study. The correlation analysis between vegetation indices calculated from spectral data and wheat growth phenotypes and yields was performed to obtain the optimal combination of vegetation indices with high correlation and good estimation performance. At the same time, the optimal model for wheat growth monitoring was screened and constructed in experiments with 12 wheat varieties and 3 gradient nitrogen fertilizer application levels. Then, the yield estimation model was established and its applicability was verified under different nitrogen fertilizer application levels. The results showed that the constructed models for the leaf area index, plant height, and yield estimation performed well, with coefficients of determination of 0.82, 0.71, and 0.70, and root mean square errors of 0.09, 2.7, and 68.5, respectively. This study provided an effective UAV remote sensing technique for monitoring wheat growth status and estimating yield. This study provides an effective unmanned aerial remote sensing technique for monitoring wheat growth and estimating yield, and provides technical support for wheat yield assessment and field management.